Scientists at Penn State University and Arizona State University, under the direction of world renowned materials scientist, Rustum Roy, PhD, have determined that the Product is unique. There are two issued patents and numerous patents pending for the Product.
Certified laboratories, university research centers and government entities have completed over fifty Product studies to date. Included are seven safety studies, antibiotic comparison studies, antibiotic additive studies, tests against thousands of different bacterium, tests against numerous viruses, and tests against yeasts and black mold. These studies include:
- Preliminary hospital-based malaria studies in Africa, wherein all patients treated with the Product became asymptomatic in an average of 5.2 days.
- Oncology clinic-based radiation burn studies in California and Arizona, wherein the Product is reducing pain and accelerating healing, at relatively low cost to the patient.
- Just-completed pig studies that demonstrated that the Product works more effectively than a specific FDA-approved, over-the-counter (“OTC”) drug for the treatment of topical wounds. The Product outperformed the OTC product by both directly killing bacteria while also stimulating the immune system, thereby compounding the healing process.
- Independent laboratory tests confirming that the Product completely kills anthrax spores and bubonic plague bacteria.
- Centers for Disease Control (“CDC”) tests showing that the Product kills SARS.
- Virology tests showing that against the Hepatitis-B Virus (“HBV”) the Product’s inhibition rate is 86.93% compared to 18.06% for AZT, a drug of choice for the treatment of HBV and HIV/AIDS.
- Spraying the Product on meat, killing 99.98% of all bacteria in two minutes, thus reducing spoilage and increasing shelf life.
- Independent tests demonstrating that the Product kills bacteria and viruses at least 200% to 300% more effectively than other silver-based product, although the other products contain hundreds of times higher concentrations of sliver.
- Safety studies demonstrating that the Product does not kill live human cells nor does it kill “friendly bacteria” in the intestinal tract (“Selective Inaction of NanoSil™ on Probiotics,” Viridus BioPharma, March 2004).
There is no comparison between NanoSil’s Silver Solutions and Other Silver Products. NanoSil silver products have been proven to kill bacteria at levels of between 2.5-5 ppm. Some silver products range between 50,000-300,000 ppm. Thus, NanoSil products are effective with concentrations of 20,000 - 60,000 times less silver in the solution. Better technology which produces a more useful (bacteria lethal) product would seem to be the difference. One can easily conclude that because NanoSil silver solution effectively kills bacteria using thousands of times less silver, the risk of any possible side effects has been eliminated.
Antimicrobial Activity of NanoSil-10 Solution
Independent University Study
Introduction:
Silver, in all its forms, has been used through the ages as an antimicrobial agent (4). Silver is known to have a broad spectrum antimicrobial activity and has been used for numerous medicinal purposes. Some of the more notable uses have been to reduce the incidence of ophthalmia neonatorum by applying silver nitrate to the eyes of newborns (7), more recently; silver in the form of silver sulphadiazine has been used for the treatment of burn wound infections (2).
The NanoSil silver solution evaluated was obtained from and tested by an independent laboratory and found to be non-toxic in an acute oral toxicity study (1,6). Colloidal silver is not new, it was used to treat a wide variety of diseases and conditions in the beginning of the 20th century. However, with the advent of antibiotics, the use of colloidal silver declined. In recent years, the use of colloidal silvers, as an antimicrobial agent, has increased because of the appearance of drug resistant strains of bacteria.
The purposes of this study were to determine the in vitro antimicrobial activity of NanoSil™ and to compare it to the activities of representative antibiotics from five classes of antibiotics-namely the tetracyclines (Tetracycline), the fluorinated quinolones (Ofloxacin), the penicillins (Penicillin G), the cephalosporins (Cefaperazone), and the macrolides (Erythromycin).
Materials and Methods:
Antimicrobials
Erythromycin (Westwood Pharmaceuticals), Ofloxacin (Sigma), Tetracycline (Sigma), Penicillin G(Sigma), and Cefaperazone (Sigma) were used. Antibiotics were diluted from stock solutions to 10 ppm (gg/m1) and used immediately for each test. The NanoSil™ silver product was used to perform the tests. Concentrations of the silver preparation were determined in an atomic absorption spectrometer (Perk and Elmers) to be 20 ppm (pg/ml).
Microorganisms
Streptococcus gordonii ATCC 10558, Streptococcus mutans ATCC 25175, Streptococcus faecalis 2-54, Streptococcus pneumoniae ATCC 6303, Streptococcus pyogenes ATCC 19615, Staphylococcus aureus (non-hemolytic) Utah Valley Regional Medical Center clinical isolate, Kiebsielia oxytoca Provo River, Utah isolate, Klebsiella pneumoniae ATCC 13883, Escherichia coil B.S.E. Luria Strain B 11303, Salmonella typhimurium ATCC 14028, Salmonella arizona C, Enterobacter cloacae Provo River, Utah isolate, Enterobacter aerogenes ATCC 13048, Shigella boydii Utah Valley Regional Medical Center clinical isolate, Pseudomonas aeruginosa ATCC 27853 were used to compare the antimicrobial activity of colloidal silver, tetracycline, erythromycin, cefaperazone, penicillin G, and ofloxacin. Streptococcal cultures were prepared by inoculating the bacteria into tryptic soy broth, TSB, (Difco) and incubating for 24 hrs. at 37C in a C02 incubator. All other bacteria were prepared by inoculating the bacteria in Mueller-Hinton Broth, MHB, (Difco) and incubating for 24 hrs. at 37C. After the 24 hour incubation period, bacteria were reinoculated and incubated until log-phase growth was reached.
Methods
The broth macrodilution susceptibility test (5) was used to determine the minimum inhibitory concentration (1WC) and the minimum bactericidal concentration (MBC) of the NanoSil preparation. This method was also used to the compare the activity of the NanoSil preparation to the antibiotics. Tests were performed by adding 1 ml of culture, (105 organisms) to two-fold serial dilution of broth containing NanoSil or antibiotic.. Tests were incubated for 24 hours and the MIC was defined as the lowest concentration of the NanoSil preparation or the antibiotic preparation that prevented turbid growth. The MBCs were demonstrated by removing a 0.1 –ml aliquot from the non-turbid tubes and inoculating tryptic soy agar (enriched with 5% sheep blood), for- streptococcal species, or Mueller-Hinton agar for all other bacteria. The MBC was defined as the lowest concentration of the NanoSil allowing the growth of fewer than 10 colonies.
Data is presented as MIC/MBC in parts per million (ppm); > denotes that the concentration needed to obtain the MIC or the MBC was higher than test- parameters measured for the test. Also, the MIC/MBC of E. coli strain 0157:H7, that has been associated with outbreaks of hemorrhagic diarrhea and colitis, was determined in a later study. The MIC was determined to be 2.5 ppm and the NBC was determined to be 5 ppm.
Conclusion
NanoSil was tested and found to be both bacteriostatic and bactericidal at 20 ppm for all organisms tested. In other studies, this product was compared to other commercially available colloidal silver products and found to have a superior activity to all other preparations tested (Revelli, unpublished data). The most interesting observation was the broad spectrum that the NanoSil possesses. The antimicrobial activity that was observed was fairly constant, independent of the particular organism tested. With the exception of S. faecalis and S. aureus (which had MIC values of 10 ppm and 5 ppm, respectively), MIC values ranged between 1.25 ppm and 2.5 ppm for both gram positive and gram negative organisms. The MBC values behaved similarly with values ranging from 1.25 ppm to 5 ppm with the exception of S. mutans, S. gordonii, and S. faecalis (which all had MBC values of 10 ppm). The data suggests that NanoSil exhibits an equal or broader spectrum of activity than any one antibiotic tested.
Antibiotics generally have restricted antibacterial spectrums limited to susceptible organisms, but as the data suggests, the NanoSil is equally effective against both gram positive and gram negative organisms.
Silver has been historically used as an antimicrobial agent (4). Metallic silver has been found to have low toxicity associated with its use especially in colloidan form (1, 6). The data suggests that with the low toxicity associated with colloidal silver in general, and the broad spectrum of antimicrobial activity of this NanoSil preparation; this preparation may be effectively used as an alternative to antibiotics. The antimicrobial activity of the colloidal silver preparation invivo could not be determined because of the nature of the tests performed. However, this is currently being studied, as are the antiviral and possible anti-inflammatory properties.
References
1. U.S. EPA IRIS Report for Silver-CASRN 7440-22-4
2. Fox CL, Modak SM.. Mechanism of Silver Sulphadiazine Action on Burn Wound Infections. Antimicrob Agents Chemother. 5:582-588.1974.
3. Furchner, JE, Richmond CR, and GA Drake. Comparative Metabolism of Radionuclides in Mammals. IV. Retention of Silver-110m in the Mouse, Rat, Monkey, and Dog. Health Phys. 15:505-514.1968.
4. Grier, N. Silver and its Compounds in Disinfection, Sterilization, and Preservation. (Seymour S. Block, ed) 2nd Edition, pp. 395-407. 1977.
5. Hindler, JA, and JH Jorgensen. Procedure in Antimicrobial Testing in Diagnostic Microbiology. (CR Mahon and G Manuselis, eds) pp 63-91. 1995.
6. NAMSA Laboratories, California. Unpublished confidential report. 1999.
7. Perrelli G, Piolatto G. Tentative Reference Values for Gold, Silver, and Platinum: Literature Data Analysis. Sci Total Environ
NanoSilTM Testing Results
The following results suggest that NanoSil is a broad spectrum antimicrobial agent and is able to effectively stop the growth of, and in fact kill, a large variety of bacteria.
NanoSil has been tested against the following organisms:
- Staphylococcus aureus (Pneumonia, eye infections, skin infections (boils, impetigo, cellulitis, and postoperative wound infections), toxic shock syndrome, meningitis, food poisoning, osteomyelitis, and many others), inhibited @ 2.5 ppm and killed @ 5 ppm 1722/99 BYU Report.
- Shiiella bovdii (Violent food poisoning-characterized by severe cramping, abdominal pain and bloody diarrhea), inhibited @ 1.25 ppm and killed @ 2.5 ppm. 1/22/99 BYU Report.
- Salmonella arizona, (Food poisoning etc.), inhibited @ 2.5 ppm and killed @ 5 ppm. 1/28/99 BYU Report.
- Salmonella tvphimurium (Food poisoning, enteric fever), inhibited and killed @ 2.5ppm. 6/7/99 BYU Report.
- E coli (Food poisoning, urinary tract (bladder) infections, diarrhea in infants, Traveler's diarrhea, respiratory tract infections, wound infections, etc.), inhibited and killed @ 2.5 ppm. 1/22/99 BYU Report.
- Haemonhiles influenzae (Otitis media (ear infections), pneumonia, meningitis, throat and sinus infections (including epiglottitis in children and sinusitis), and suppurative arthritis in children), inhibited and killed @ 1.25 ppm. 1/22/99 BYU Report.
- Klebsiella pneumoniae (lower respiratory tract infections (i.e. pneumonia), meningitis, nosocomial infections-infections spread in hospitals, urinary tract and wound infections, and bacteremia), inhibited and killed @ 2.5 ppm. 1/28/99 BYU Report.
- Klebsiella oxytoca (Similar to those infections caused by K. pneumoniae), killed in water treatment tests @ 0.1 ppm. 3/15/99 CUWCD Report. Also it was inhibited and killed @ 2.5ppm. 6/7/99 BYU Report.
- Enterobacter aerogenes (wound infections, urinary tract infections, bacteremia, and meningitis), inhibited and killed @ 2.5ppm. 6/7/99 BYU Report.
- Enterobacter cloacae (causes illnesses similar to E. aerogenes), was inhibited and killed @ 5ppm. 6/7/99 BYU Report.
- Pseudomonas aeruginosa ( severe burn and wound infections, keratitis, meningitis, pneumonia, nosocomial infections, urinary tract infections, etc.), inhibited @ 2.5 ppm and killed @ 5 ppm. 1/22/99 BYU Report.
- Streptococcus pneumoniae (Pneumonia, meningitis, sinusitis, otitis media-ear infections), inhibited @ 2.5 ppm and killed @ 5 ppm. 4/21/99 BYU Report.
- Streptococcus pvoienes, (skin infections, upper respiratory infections (Le. strep throat), impetigo, hospital-acquired infections, scarlet fever, etc.), inhibited and killed @ 1.25 ppm. 1/22/99 BYU Report.
- Streptococcus faecalis (Urinary tract infections, endocarditis, wound infections, etc.), inhibited @ 2.5 ppm and killed @ 5 ppm. 1/22/99 BYU Report.
- Streptococcus mutans, (A major cause of dental plaque and tooth decay etc.), inhibited and killed @ 5 ppm. 2/3/99 BYU Report.
- Streptococcus Rordonii (Tooth decay, also implicated jn infective endocarditis-- infection of the heart valves), inhibited and killed @ 5 ppm. BYU Report 2112/99.
NanoSil™ Spray Gel
Nano-Silver Topical Anti-Microbial Spray
DESCRIPTION: NanoSil™ Spray Gel is a unique penetrating antibiotic gel for treating the pain, inflammation and other pathological conditions affecting musculoskeletal tissues and other soft tissues of the body caused by microbial infections. NanoSil™ Spray Gel is based on the historical anti-microbial characteristics of NanoSil™-10 Solution now in a novel organogel compound whose unique three-dimensional network provides excellent suspension functionality and creates a thixotropic characteristic providing a pourable liquid that reforms a gel when applied providing for extended dwell time allowing it to be used in an easy to apply spray with an unequalled degree of shear thinning and the reformation of a non-elastic highly thixotropic gel , making topical applications allowing improved penetration of sub-dermal soft tissues possible.
Uses: All topical wounds and abrasions.
Features:
- True nano-phase technology. Particle size smaller than 25 nm
- No added thickeners, gel formation by natural hydro-swelling of mineral particulates.
- Patent-pending silver particle formation
- Novel shearing gel
- Aids in wound healing
- Natural mineral contents aid in tissue repair
- Novel spraying gel
Benefits:
- Drug free anti-microbial
- Child safe
Remarks: Unique water-based thixotropic gel vehicle provides better penetration and reduced irritation as compared to alcohol or competitive water-based gel products.